Adipocyte & Metabolic Models
In diet-induced obese mouse models, white adipose tissue mass ~35% below control was recorded following NNMT inhibition with 5-Amino-1MQ, with food intake unchanged - pointing to energy expenditure rather than appetite.
≥99% Purity
5-Amino-1MQ
NNMT Inhibition Studies
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About this compound
5-Amino-1MQ is a small molecule rather than a peptide. It blocks an enzyme called NNMT, which consumes nicotinamide, a building block cells use to make NAD+. Studies have measured what happens to NAD+ levels and fat cell metabolism when that enzyme is blocked. Unlike most compounds in this catalog, it survives being taken by mouth.
Small-molecule NNMT inhibitor (5-amino-1-methylquinolinium)
- Formula
- C10H10N2
- Molecular weight
- 158.19 g/mol
- Form
- Lyophilized powder
- CAS / ID
- 4213-45-0
0%
Fat Mass Reduction
White adipose tissue in DIO mouse models
0.0×
NAD+ Elevation
Intracellular NAD+ (fold change)
0
Research Areas
Adipocyte & Metabolic Models · NAD+ Salvage Pathway · SAM Methyl Donor Pool & more
How It Works
How 5-Amino-1MQ works
The pathways 5-Amino-1MQ acts on — and what each one does. The animation traces its signal outward from the compound to every target it engages.
Signalling pathways studied in preclinical models · illustrative
Nicotinamide N-Methyltransferase Blockade
NNMT Inhibition
5-Amino-1MQ is a small-molecule competitive inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes the methylation of nicotinamide using S-adenosyl methionine (SAM). NNMT is overexpressed in adipose tissue of obese subjects, and its inhibition has been explored as a strategy to modulate methyl group flux and NAD+ precursor availability.
- Competitive inhibitor of NNMT enzyme activity
- Lower 1-methylnicotinamide (MNA) production measured
- Preserves SAM for other methylation reactions
NAD+ Precursor Salvage Pathway Modulation
NAD+ Metabolism
By blocking NNMT-mediated consumption of nicotinamide, 5-Amino-1MQ redirects nicotinamide back into the NAD+ salvage pathway. Preclinical studies demonstrate elevated intracellular NAD+ levels following NNMT inhibition, suggesting a role in metabolic energy regulation.
- Redirects nicotinamide into NAD+ salvage pathway
- Elevates intracellular NAD+ (~1.6× in preclinical models)
- Modulates NAMPT-dependent NAD+ biosynthesis
Adipocyte Energy Metabolism
Adipocyte Metabolism
In diet-induced obese (DIO) mouse models, NNMT inhibition with 5-Amino-1MQ was associated with reduced white adipose tissue mass without affecting food intake, suggesting changes in energy expenditure rather than appetite suppression. Researchers observed upregulation of metabolic gene expression in adipocytes.
- Reduced white adipose tissue mass in DIO mouse models
- No change in food intake observed
- Altered adipocyte gene expression patterns
Uses & Applications
What 5-Amino-1MQ is researched for
The main areas 5-Amino-1MQ is being studied for — and the study-reported figures behind them.
NAD+ Salvage Pathway
5-Amino-1MQ preserves nicotinamide for NAD+ biosynthesis by blocking NNMT-catalyzed methylation, elevating intracellular NAD+ in adipocyte cell lines.
SAM Methyl Donor Pool
NNMT consumes SAM as a methyl donor, competing with DNA and histone methyltransferases. NNMT inhibition may influence epigenetic programming in metabolic tissues.
Glucose Uptake Models
NNMT knockdown in adipocytes was associated with changes in insulin sensitivity and glucose uptake markers in preclinical in vitro models, which is why NNMT is studied in metabolic regulation.
Molecular Structure
The 5-Amino-1MQ molecule
An interactive 3D model rendered from the compound record — rotate and explore its structure.
C10H10N2
Compound Information
Full specification
| Chemical Name | 5-Amino-1-methylquinolinium |
| CAS Number | 4213-45-0 |
| Molecular Weight | 158.19 Da |
| Molecular Formula | C₁₀H₁₀N₂ |
| Target | Nicotinamide N-methyltransferase (NNMT) |
| Mechanism | Competitive NNMT enzyme inhibitor |
| Form | Lyophilized powder |
| Purity | ≥99% (HPLC verified) |
| Testing | Third-party HPLC, Mass Spec, Endotoxin |
| Storage | -20°C, protect from light |
| COA | Included with every order |
FAQ
Common questions about 5-Amino-1MQ
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide using S-adenosyl methionine (SAM). NNMT is upregulated in adipose tissue in obesity, and its inhibition is studied as a potential strategy to modulate NAD+ metabolism and metabolic gene expression. It is used exclusively as a research tool compound.
NNMT metabolizes nicotinamide into 1-methylnicotinamide (MNA), diverting it away from the NAD+ salvage pathway. When NNMT is inhibited by compounds like 5-Amino-1MQ, more nicotinamide becomes available for conversion to NAD+ via NAMPT. Preclinical studies report approximately 1.6× increases in intracellular NAD+ following NNMT inhibition in adipocyte models.
In diet-induced obese (DIO) mouse models, NNMT inhibition led to approximately 35% reduction in white adipose tissue mass compared to controls. Importantly, food intake remained unchanged in treated animals, suggesting the compound's effects relate to energy expenditure pathways rather than appetite. These findings have not been replicated in human trials.
No. 5-Amino-1MQ is a mechanistically distinct research compound. Rather than directly supplying NAD+ precursors (like NMN or NR), it works by blocking an enzyme that consumes nicotinamide, theoretically preserving the substrate pool for endogenous NAD+ production. Its effects on overall NAD+ biology are still being characterized in preclinical research.
No. 5-Amino-1MQ has not been approved by the FDA or any regulatory agency for human use. No human clinical trials have been completed. It is sold exclusively as a research tool compound for in vitro laboratory use and is not intended for human consumption.
Store lyophilized 5-Amino-1MQ at -20°C and protect from light. For in vitro studies, it is typically dissolved in DMSO or aqueous buffer depending on the experimental protocol.
Research Use Only.
Not for human or veterinary use. For in-vitro laboratory research only. These statements have not been evaluated by the FDA; this product is not intended to diagnose, treat, cure, or prevent any disease. Sold exclusively to qualified researchers and institutions.